Evaluation of Fluorescently Labeled Aerolysin as a New Kind of Reagent for Flow Cytometry Tests
نویسندگان
چکیده
منابع مشابه
Simplified protocol for flow cytometry analysis of fluorescently labeled exosomes and microvesicles using dedicated flow cytometer
Flow cytometry is a powerful method, which is widely used for high-throughput quantitative and qualitative analysis of cells. However, its straightforward applicability for extracellular vesicles (EVs) and mainly exosomes is hampered by several challenges, reflecting mostly the small size of these vesicles (exosomes: ~80-200 nm, microvesicles: ~200-1,000 nm), their polydispersity, and low refra...
متن کاملFlow Cytometry: A New Approach for Indirect Assessment of Sperm Protamine Deficiency
Background Flow cytometry (FCM) has been extensively used to study mammalian sperm in the areas of clinical andrology and reproductive toxicology. FCM provides a powerful advantage over microscopy technique in terms of rapid accurate and reproducible technology for the quantification of various cell characteristics including chromatin status. During spermiogenesis histones are replaced by prota...
متن کاملassessment of the effect of honey as a topical therapy for intra oral wound healing in rat.
چکیده ندارد.
15 صفحه اولEvaluation of a new bombesin analogue labeled with 99mTc as potential targeted tumor scintigraphic agent
Background: Bombesin shows high affinity for Gastrin-releasing peptide (GRP) receptors which over expressed on the cell surfaces of several human tumors particularly in prostate and breast cancers. The aim of this study was labeling of designed analogue with99mTc via HYNIC and Tricine /EDDA and evaluation as potential targeted tumor scintigraphic agent. Materials and Methods: HYNIC-Bombesin wa...
متن کاملFluorescently labeled ribosomes as a tool for analyzing antibiotic binding.
Measuring the binding of antibiotics and other small-molecular-weight ligands to the 2.5 MDa ribosome often presents formidable challenges. Here, we describe a general method for studying binding of ligands to ribosomes that carry a site-specific fluorescent label covalently attached to one of the ribosomal proteins. As a proof of principle, an environment-sensitive fluorescent group was placed...
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ژورنال
عنوان ژورنال: American Journal of Clinical Pathology
سال: 2016
ISSN: 0002-9173,1943-7722
DOI: 10.1093/ajcp/aqv096